Variant Calling (Part 2): From Bash to Nextflow: GATK Best Practice With Nextflow
In Part 1, we built a complete 10-step GATK variant calling pipeline in bash—perfect for academic research and 1-10 samples. But what happens when you need to scale to 100+ samples? This is where Nextflow becomes essential.
📁 Repository: All code from this tutorial is organized in the nf-germline-short-read-variant-calling repository. The structure follows best practices with separate directories for bash (
bash-gatk) and Nextflow (nextflow-gatk) implementations.