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Variant Calling (Part 2): From Bash to Nextflow: GATK Best Practice With Nextflow

· 27 min read
Thanh-Giang Tan Nguyen
Founder at RIVER

In Part 1, we built a complete 10-step GATK variant calling pipeline in bash—perfect for academic research and 1-10 samples. But what happens when you need to scale to 100+ samples? This is where Nextflow becomes essential.

📁 Repository: All code from this tutorial is organized in the nf-germline-short-read-variant-calling repository. The structure follows best practices with separate directories for bash (bash-gatk) and Nextflow (nextflow-gatk) implementations.

How to Migrate from In-House Pipelines to Enterprise-Level Workflows: A Proven 3-Step Validation Framework

· 18 min read
Thanh-Giang Tan Nguyen
Founder at RIVER

Whether your lab uses bash scripts, Python workflows, Snakemake pipelines, or custom solutions—your in-house pipeline works fine locally. It's been running for years. But as your research scales, you face a hard truth: in-house pipelines don't scale, aren't reproducible across teams, and require constant manual fixes.